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Acidic‐pH‐Activatable Fluorescence Probes for Visualizing Exocytosis Dynamics
Author(s) -
Asanuma Daisuke,
Takaoka Yousuke,
Namiki Shigeyuki,
Takikawa Kenji,
Kamiya Mako,
Nagano Tetsuo,
Urano Yasuteru,
Hirose Kenzo
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201402030
Subject(s) - exocytosis , endocytosis , fluorescence , chemistry , nanotechnology , biophysics , live cell imaging , secretion , biological system , materials science , biochemistry , cell , biology , physics , quantum mechanics
Live imaging of exocytosis dynamics is crucial for a precise spatiotemporal understanding of secretion phenomena, but current approaches have serious limitations. We designed and synthesized small‐molecular fluorescent probes that were chemically optimized for sensing acidic intravesicular pH values, and established that they can be used to sensitively and reliably visualize vesicular dynamics following stimulation. This straightforward technique for the visualization of exocytosis as well as endocytosis/reacidification processes with high spatiotemporal precision is expected to be a powerful tool for investigating dynamic cellular phenomena involving changes in the pH value.