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Reversible Re‐programing of Cell–Cell Interactions
Author(s) -
Gabrielse Kari,
Gangar Amit,
Kumar Nigam,
Lee Jae Chul,
Fegan Adrian,
Shen Jing Jing,
Li Qing,
Vallera Daniel,
Wagner Carston R.
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201310645
Subject(s) - cell , chimeric antigen receptor , peripheral blood mononuclear cell , chemistry , cancer cell , microbiology and biotechnology , receptor , biophysics , ligand (biochemistry) , nanotechnology , materials science , t cell , biochemistry , biology , cancer , in vitro , immunology , immune system , genetics
The ability to engineer and re‐program the surfaces of cells would provide an enabling synthetic biological method for the design of cell‐ and tissue‐based therapies. A new cell surface‐engineering strategy is described that uses lipid‐chemically self‐assembled nanorings (lipid‐CSANs) that can be used for the stable and reversible modification of any cell surface with a molecular reporter or targeting ligand. In the presence of a non‐toxic FDA‐approved drug, the nanorings were quickly disassembled and the cell–cell interactions reversed. Similar to T‐cells genetically engineered to express chimeric antigen receptors (CARS), when activated peripheral blood mononuclear cells (PBMCs) were functionalized with the anti‐EpCAM‐lipid‐CSANs, they were shown to selectively kill antigen‐positive cancer cells. Taken together, these results demonstrate that lipid‐CSANs have the potential to be a rapid, stable, and general method for the reversible engineering of cell surfaces and cell–cell interactions.