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Rare‐Cell Enrichment by a Rapid, Label‐Free, Ultrasonic Isopycnic Technique for Medical Diagnostics
Author(s) -
Bourquin Yannyk,
Syed Abeer,
Reboud Julien,
RanfordCartwright Lisa C.,
Barrett Michael P.,
Cooper Jonathan M.
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201310401
Subject(s) - parasitemia , ultrasonic sensor , blood cell , cell , malaria , biomedical engineering , chemistry , materials science , immunology , biology , medicine , biochemistry , plasmodium falciparum , radiology
One significant challenge in medical diagnostics lies in the development of label‐free methods to separate different cells within complex biological samples. Here we demonstrate a generic, low‐power ultrasonic separation technique, able to enrich different cell types based upon their physical properties. For malaria, we differentiate between infected and non‐infected red blood cells in a fingerprick‐sized drop of blood. We are able to achieve an enrichment of circulating cells infected by the ring stage of the parasite over nonparasitized red blood cells by between two and three orders of magnitude in less than 3 seconds (enabling detection at parasitemia levels as low as 0.0005 %). In a second example, we also show that our methods can be used to enrich different cell types, concentrating Trypanosoma in blood at very low levels of infection, on disposable, low‐cost chips.