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Irreversible Site‐Specific Hydrazinolysis of Proteins by Use of Sortase
Author(s) -
Li YiMing,
Li YiTong,
Pan Man,
Kong XiuQi,
Huang YiChao,
Hong ZhangYong,
Liu Lei
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201310010
Subject(s) - semisynthesis , sortase , chemistry , recombinant dna , thioester , fusion protein , sortase a , combinatorial chemistry , hydrazine (antidepressant) , biochemistry , bacterial protein , enzyme , gene
Sortase‐mediated hydrazinolysis of proteins with hydrazine or its derivatives was developed for the production of recombinant protein hydrazides. This process provides an alternative approach for protein semisynthesis through the use of recombinant protein hydrazides as thioester surrogates. It also provides an alternative method for C‐terminal modification of proteins with functional units as well as for the preparation of C‐to‐C fusion proteins.