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Regulation of the Cyanobacterial Circadian Clock by Electrochemically Controlled Extracellular Electron Transfer
Author(s) -
Lu Yue,
Nishio Koichi,
Matsuda Shoichi,
Toshima Yuki,
Ito Hiroshi,
Konno Tomohiro,
Ishihara Kazuhiko,
Kato Souichiro,
Hashimoto Kazuhito,
Nakanishi Shuji
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201309560
Subject(s) - redox , extracellular , circadian clock , electron transfer , circadian rhythm , biophysics , chemistry , intracellular , microbiology and biotechnology , photochemistry , biochemistry , biology , neuroscience , inorganic chemistry
There is growing awareness that circadian clocks are closely related to the intracellular redox state across a range of species. As the redox state is determined by the exchange of the redox species, electrochemically controlled extracellular electron transfer (EC‐EET), a process in which intracellular electrons are exchanged with extracellular electrodes, is a promising approach for the external regulation of circadian clocks. Herein, we discuss whether the circadian clock can be regulated by EC‐EET using the cyanobacterium Synechococcus elongatus PCC7942 as a model system. In vivo monitoring of chlorophyll fluorescence revealed that the redox state of the plastoquionone pool could be controlled with EC‐EET by simply changing the electrode potential. As a result, the endogenous circadian clock of S. elongatus cells was successfully entrained through periodically modulated EC‐EET by emulating the natural light/dark cycle, even under constant illumination conditions. This is the first example of regulating the biological clock by electrochemistry.