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Color‐Discriminating Retinal Configurations of Sensory Rhodopsin I by Photo‐Irradiation Solid‐State NMR Spectroscopy
Author(s) -
Yomoda Hiroki,
Makino Yoshiteru,
Tomonaga Yuya,
Hidaka Tetsurou,
Kawamura Izuru,
Okitsu Takashi,
Wada Akimori,
Sudo Yuki,
Naito Akira
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201309258
Subject(s) - rhodopsin , phototaxis , photochemistry , spectroscopy , chemistry , irradiation , nuclear magnetic resonance spectroscopy , photobiology , retinal , pigment , stereochemistry , optics , organic chemistry , botany , biology , physics , biochemistry , quantum mechanics , nuclear physics
SRI (sensory rhodopsin I) can discriminate multiple colors for the attractant and repellent phototaxis. Studies aimed at revealing the color‐dependent mechanism show that SRI is a challenging system not only in photobiology but also in photochemistry. During the photoreaction of SRI, an M‐intermediate (attractant) transforms into a P‐intermediate (repellent) by absorbing blue light. Consequently, SRI then cycles back to the G‐state. The photoreactions were monitored with the 13 C NMR signals of [20‐ 13 C]retnal‐ Sr SRI using in situ photo‐irradiation solid‐state NMR spectroscopy. The M‐intermediate was trapped at −40 °C by illumination at 520 nm. It was transformed into the P‐intermediate by subsequent illumination at 365 nm. These results reveal that the G‐state could be directly transformed to the P‐intermediate by illumination at 365 nm. Thus, the stationary trapped M‐ and P‐intermediates are responsible for positive and negative phototaxis, respectively.