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Real‐Time Monitoring of Auxin Vesicular Exocytotic Efflux from Single Plant Protoplasts by Amperometry at Microelectrodes Decorated with Nanowires
Author(s) -
Liu JunTao,
Hu LiangSheng,
Liu YanLing,
Chen RongSheng,
Cheng Zhi,
Chen ShiJing,
Amatore Christian,
Huang WeiHua,
Huo KaiFu
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201308972
Subject(s) - amperometry , exocytosis , auxin , microelectrode , protoplast , chemistry , efflux , biophysics , nanotechnology , electrochemistry , biochemistry , membrane , biology , materials science , electrode , gene
Recent biochemical results suggest that auxin (IAA) efflux is mediated by a vesicular cycling mechanism, but no direct detection of vesicular IAA release from single plant cells in real‐time has been possible up to now. A TiC@C/Pt‐QANFA micro‐electrochemical sensor has been developed with high sensitivity in detection of IAA, and it allows real‐time monitoring and quantification of the quantal release of auxin from single plant protoplast by exocytosis.