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Single Mutations in Tau Modulate the Populations of Fibril Conformers through Seed Selection
Author(s) -
Meyer Virginia,
Dinkel Paul D.,
Luo Yin,
Yu Xiang,
Wei Guanghong,
Zheng Jie,
Eaton Gareth R.,
Ma Buyong,
Nussinov Ruth,
Eaton Sandra S.,
Margittai Martin
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201308473
Subject(s) - fibril , conformational isomerism , tau protein , mutant , chemistry , biophysics , gene isoform , point mutation , crystallography , biochemistry , alzheimer's disease , biology , molecule , gene , disease , medicine , organic chemistry , pathology
Seeded conversion of tau monomers into fibrils is a central step in the progression of tau pathology in Alzheimer’s disease and other neurodegenerative disorders. Self‐assembly is mediated by the microtubule binding repeats in tau. There are either three or four repeats present depending on the protein isoform. Here, double electron‐electron resonance spectroscopy was used to investigate the conformational ensemble of four‐repeat tau fibrils. Single point mutations at key positions in the protein (ΔK280, P301S, P312I, D314I) markedly change the distribution of fibril conformers after template‐assisted growth, whereas other mutations in the protein (I308M, S320F, G323I, G326I, Q336R) do not. These findings provide unprecedented insights into the seed selection of tau disease mutants and establish conformational compatibility as an important driving force in tau fibril propagation.