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An Atypical Naturally Split Intein Engineered for Highly Efficient Protein Labeling
Author(s) -
Thiel Ilka V.,
Volkmann Gerrit,
Pietrokovski Shmuel,
Mootz Henning D.
Publication year - 2014
Publication title -
angewandte chemie
Language(s) - English
Resource type - Journals
eISSN - 1521-3757
pISSN - 0044-8249
DOI - 10.1002/ange.201307969
Subject(s) - intein , protein splicing , protein evolution , protein engineering , protein tag , rna splicing , chemical biology , chemistry , synthetic biology , amino acid , peptide , trans splicing , biochemistry , computational biology , directed evolution , protein design , metagenomics , combinatorial chemistry , mutant , biology , protein structure , gene , enzyme , fusion protein , recombinant dna , rna
Protein trans‐splicing catalyzed by split inteins is a powerful technique for assembling a polypeptide backbone from two separate parts. However, split inteins with robust efficiencies and short fragments suitable for peptide synthesis are rare and have mostly been artificially created. The novel split intein AceL‐TerL was identified from metagenomic data and characterized. It represents the first naturally occurring, atypically split intein. The N‐terminal fragment of only 25 amino acids is the shortest natural intein fragment to date and was easily amenable to chemical synthesis with a fluorescent label. Optimal protein trans‐splicing activity was observed at low temperatures. Further improved mutants were selected by directed protein evolution. The engineered intein variants with up to 50‐fold increased rates showed unprecedented efficiency in chemically labeling of a diverse set of proteins. These inteins should prove valuable tools for protein semi‐synthesis and other intein‐related biotechnological applications.