Open AccessParameters Influencing Gene Delivery Efficiency of PEGylated Chitosan Nanoparticles: Experimental and Modeling Approach
Author(s) -
Dogan Nihal Olcay,
Bozuyuk Ugur,
Erkoc Pelin,
Karacakol Alp Can,
Cingoz Ahmet,
Seker-Polat Fidan,
Nazeer Muhammad Anwaar,
Sitti Metin,
Bagci-Onder Tugba,
Kizilel Seda
Publication year - 2022
Publication title -
advanced nanobiomed research
Language(s) - English
Resource type - Journals
ISSN - 2699-9307
DOI - 10.1002/anbr.202100033
Subject(s) - polyethylene glycol , nanomedicine , nanoparticle , chitosan , transfection , peg ratio , gene delivery , artificial neural network , biological system , nanotechnology , computer science , materials science , chemistry , artificial intelligence , gene , biology , biochemistry , finance , economics
Experimentation of nanomedicine is labor‐intensive, time‐consuming, and requires costly laboratory consumables. Constructing a reliable mathematical model for such systems is also challenging due to the difficulties in gathering a sufficient number of data points. Artificial neural networks (ANNs) are indicated as an efficient approach in nanomedicine to investigate the cause‐effect relationships and predict output variables. Herein, an ANN is adapted into plasmid DNA (pDNA) encapsulated and PEGylated chitosan nanoparticles cross‐linked with sodium tripolyphosphate (TPP) to investigate the effects of critical parameters on the transfection efficiencies of nanoparticles. The ANN model is developed based on experimental results with three independent input variables: 1) polyethylene glycol (PEG) molecular weight, 2) PEG concentration, and 3) nanoparticle concentration, along with one output variable as a percentage of green fluorescent protein (GFP) expression, which refers to transfection efficiency. The constructed model is further validated with the leave‐p‐out cross‐validation method. The results indicate that the developed model has good prediction capability and is influential in capturing the transfection efficiencies of different nanoparticle groups. Overall, this study reveals that the ANN could be an efficient tool for nanoparticle‐mediated gene delivery systems to investigate the impacts of critical parameters in detail with reduced experimental effort and cost.