Premium
Hu antigens: Reactivity with hu antibodies, tumor expression, and major immunogenic sites
Author(s) -
Manley Geoffrey T.,
Smitt Peter Sillevis,
Dalmau Josep,
Posner Jerome B.
Publication year - 1995
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410380117
Subject(s) - antigen , encephalomyelitis , antibody , epitope , immunology , immune system , medicine , biology , multiple sclerosis
HuD, a human neuronal RNA‐binding protein, was the first identified member of a family of antigens that also includes HuC and Hel‐N1 (Hu antigens). The serum of all patients with anti‐Hu–associated paraneoplastic encephalomyelitis and sensory neuronopathy react with HuD and Hel‐N1, but the reactivity with HuC is unknown. In the current study we examined (1) the reactivity of anti‐Hu sera with HuD, HuC, and Hel‐N1; (2) the expression of HuD, HuC, and Hel‐N1 messenger RNA in small‐cell lung cancer of patients with and those without paraneoplastic encephalomyelitis/sensory neuronopathy; (3) the correlation between anti‐Hu serum reactivity with these three Hu antigens and the type of neurological symptoms; and (4) the major immunogenic sites of HuD. Our findings indicate that all anti‐Hu sera react with HuD, HuC, and Hel‐N1. However, only HuD is expressed in the small‐cell lung cancer tumors, indicating that among the three Hu antigens, HuD appears to play a central role in triggering the anti‐Hu immune response. No differences were identified regarding the reactivity of the anti‐Hu antibodies with HuD, HuC, and Hel‐N1 and the spectrum of neurological symptoms presented by the patients. Study of the major immunogenic sites of HuD resulted in the identification of two major immunodominant regions with at least two distinct epitopes recognized by the serum of all patients with anti‐Hu—associated paraneoplastic encephalomyelitis/sensory neuronopathy.