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Localization of non– N ‐methyl‐ D ‐aspartate glutamate receptors in normal and Alzheimer hippocampal formation
Author(s) -
Hyman Bradley T.,
Penney John B.,
Blackstone Craig D.,
Young Anne B.
Publication year - 1994
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410350106
Subject(s) - hippocampal formation , glutamate receptor , neuroscience , dentate gyrus , alzheimer's disease , hippocampus , receptor , senile plaques , antibody , ampa receptor , biology , chemistry , pathology , biochemistry , medicine , immunology , disease
The hippocampi and adjacent temporal cortices of 24 human brains were examined with antibodies to the GluR1, GluR2/3, and GluR4 subunits of the D , L ‐α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid–preferring glutamate receptor. GluR1 immunoreactivity was most dense in the dentate gyrus, with lower densities in other hippocampal and cortical regions. GluR2/3 immunoreactivity was the most intense of the three antibodies, with high levels throughout most hippocampal subfields, where it was localized to cell bodies, proximal axons, and dendrites. GluR4 immunoreactivity was very sparse in all regions. In Alzheimer's disease brains, the general pattern of staining was similar to that seen in control brains. GluR1 and GluR4 immunoreactivity was seen in some but not all neuritic plaques. All three antibodies recognized some neurons undergoing neurofibrillary degeneration.