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Assessment of reliability and biological significance of glutamate levels in cerebrospinal fluid
Author(s) -
Ferrarese Carlo,
Pecora Nicoletta,
Frigo Maura,
Appollobnio Ildebrando,
Frattola Lodovico
Publication year - 1993
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410330316
Subject(s) - cerebrospinal fluid , glutamate receptor , medicine , receptor
The published information on glutamate levels in cerebrospinal fluid (CSF) and modifications in neurological disorders is controversial. In the present study, we demonstrated a metabolic instability of glutamare in untreated CSF and a spurious elevation of its levels by the current methods of CSF acidification. These findings may explain the discrepancies observed with different methods of CSF processing and analysis. We suggest a method of inactivating CSF enzymes that yields stable glutamate levels under different storage conditions. Use of such a method may be necessary for clinical studies.