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Lack of JC viral genomic sequences in multiple sclerosis brain tissue by polymerase chain reaction
Author(s) -
Buckle Guy J.,
Godec Mark S.,
Rubi Jorge U.,
Tornatore Carlo,
Major Eugene O.,
Gibbs Clarence J.,
Gajdusek D. Carleton,
Asher David M.
Publication year - 1992
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410320622
Subject(s) - progressive multifocal leukoencephalopathy , jc virus , polymerase chain reaction , slow virus , primer (cosmetics) , multiple sclerosis , pathology , nested polymerase chain reaction , leukoencephalopathy , medicine , virology , virus , biology , disease , immunology , gene , chemistry , genetics , organic chemistry
With DNA extracted from brain specimens from 19 multiple sclerosis, 5 progressive multifocal leukoencephalopathy, 1 Alzheimer's disease, and 8 nonneurological control subjects, polymerase chain reaction was performed using nested sets of primer pairs amplifying segments of the large T and VP1 antigen‐encoding sequences of JC virus. Both sequences were detected in each of the 5 brain specimens of progressive multifocal leukoencephalopathy but in none of the 19 multiple sclerosis, 1 Alzheimer's disease, or the 8 control brain specimens.