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Altered excitatory and inhibitory amino acid receptor binding in hippocampus of patients with temporal lobe epilepsy
Author(s) -
McDonald John W.,
Garofalo Elizabeth A.,
Hood Terry,
Sackellares J. Chris,
Gilman Sid,
McKeever Paul E.,
Troncoso Juan C.,
Johnston Michael V.
Publication year - 1991
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410290513
Subject(s) - inhibitory postsynaptic potential , gabaa receptor , excitatory postsynaptic potential , dentate gyrus , hippocampal formation , phencyclidine , hippocampus , epilepsy , temporal lobe , receptor , nmda receptor , glycine receptor , chemistry , medicine , neuroscience , endocrinology , glycine , biology , biochemistry , amino acid
Abstract We examined binding to excitatory amino acid and inhibitory amino acid receptors in frozen hippocampal sections prepared from surgical specimens resected from 8 individuals with medically refractory temporal lobe epilepsy. The excitatory receptors studied included N ‐methyl‐D‐aspartate (NMDA), strychnine‐insensitive glycine, phencyclidine, and quisqualate. The inhibitory receptors studied were gamma‐aminobutyric acid type A (GABA A ) and benzodiazepine. Excitatory and inhibitory amino acid receptor binding were differentially altered in the patients with temporal lobe epilepsy in comparison to 8 age‐comparable autopsy control subjects, and changes in receptor binding were regionally selective in four areas. Binding to phencyclidine receptors associated with the NMDA channel was reduced by 35 to 70% in all regions in the hippocampi of the patients. In contrast, binding to the NMDA recognition site and its associated glycine modulatory site was elevated by 20 to 110% in the cornu ammonis (CA) 1 area and dentate gyrus of the hippocampus of the patients. Binding to these sites was unaffected in area CA4. Binding to the quisqualate‐type excitatory amino acid receptor was unchanged in all regions except the stratum lacunosum moleculare CA1, where it was increased by 63%. GABA A and benzodiazepine receptor binding was reduced by 20 to 60% in CA1 and CA4, but unchanged in dentate gyrus. The data indicate that excitatory and inhibitory amino acid receptors are altered in the hippocampus of patients with temporal lobe epilepsy.