Premium
JC virus detection by in situ hybridization in brain tissue from elderly patients
Author(s) -
Mori Mayumi,
Kurata Hirokazu,
Tajima Masako,
Shimada Hiroyuki
Publication year - 1991
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410290414
Subject(s) - in situ hybridization , capsid , polyclonal antibodies , biotinylation , immunohistochemistry , horseradish peroxidase , virology , microbiology and biotechnology , biology , virus , viral protein , antibody , cytochemistry , dna , hybridization probe , digoxigenin , pathology , ultrastructure , medicine , messenger rna , enzyme , immunology , biochemistry , anatomy , gene
Brains from 10 patients aged 68 to 96 years at time of death were studied for JC viral DNA and common papovaviral capsid protein. In situ hybridization to JC viral DNA was performed by affinity cytochemistry using a biotinylated fragment of JC viral DNA. Immunohistochemistry was performed on brain tissue by the avidin DH‐biotinylated horseradish peroxidase technique using polyclonal antibody raised against the papovaviral capsid protein. Viral protein and DNA were detected in 4 of 10 patients. JC virus may be present in the brains of aged patients more frequently than previously suspected.