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General assay for phosphoproteins in cerebrospinal fluid: A candidate market for paraneoplastic cerebellar degeneration
Author(s) -
Gandy Samuel E.,
Grebb Jack A.,
Rosen Neal,
Albert Katherine A.,
Devinsky Orrin,
Blumberg Hilary,
Anderson Neil,
Cedarbaum Jesse M.,
Porter Roger J.,
Sedvall Göran,
Posner Jerome B.,
Greengard Paul
Publication year - 1990
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410280616
Subject(s) - cerebrospinal fluid , phosphoprotein , phosphorylation , kinase , paraneoplastic cerebellar degeneration , protein kinase a , phosphatase , pathology , 14 3 3 protein , biology , biochemistry , chemistry , microbiology and biotechnology , medicine , immunology , antibody , autoantibody
The components of protein phosphorylation systems (protein kinases, protein phosphatases, and their phosphoprotein substrates) are highly enriched in neuronal cells compared with other cell types. We exploited this relative neuronal enrichment of protein phosphorylation system components to develop a general assay technique for putative protein kinase substrates (phosphoproteins) in human cerebrospinal fluid. Using this cerebrospinal fluid phosphoprotein assay, we have detected a putative protein kinase C substrate protein of apparent M r 60 kd in 6 of 14 patients with paraneoplastic cerebellar degeneration but not in any of 55 patients with a variety of other neurological diseases. Phosphoproteins in cerebrospinal fluid may provide novel and unique markers for the diagnosis or staging of neuronal diseases as well as offer potential insights into the biochemical characterization of affected neuronal populations.