Premium
Neural transplantation using temperaturesensitive immortalized neural cells: A preliminary report
Author(s) -
Bredesen Dale E.,
Hisanaga Kinya,
Sharsp Frank R.
Publication year - 1990
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410270218
Subject(s) - immortalised cell line , transplantation , cerebellum , cell culture , neuroscience , biology , neural stem cell , microbiology and biotechnology , medicine , stem cell , genetics
Current approaches to neural transplantation–adrenal medullary and fetal brain–suffer from limitations in tissue availability, cellular uniformity, and general applicability. The system described here uses temperature‐sensitive immortalized clonal neural cells to address these limitations. Cerebellar cells, immortalized with a temperature‐sensitive tumor virus, are transformed at 34°C, but differentiate at 38°C. The cells were genetically engineered to express beta‐galactosidase, providing a tag to study their fate in the host. The cells were then successfully transplanted to the cerebellum of adult rats.