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Tau in situ hybridization in normal and alzheimer brain: Localization in the somatodendritic compartment
Author(s) -
Kosik Kenneth S.,
Crandall James E.,
Mufson Elliott J.,
Neve Rachael L.
Publication year - 1989
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410260308
Subject(s) - in situ hybridization , microbiology and biotechnology , ribosomal rna , rna , biology , messenger rna , biophysics , chemistry , biochemistry , gene
Tau messenger RNA in situ hybridization in human postmortem brain revealed that neurons are the predominant cell type labeled. The probe used includes the nucleotide sequence coding for the amino acids recognized by the well‐characterized tau monoclonal antibodies 5E2 and tau 1. The distribution of the tau RNA is abundant throughout the neuronal somata and into the proximal parts of dendrites of pyramidal cells in the cerebral cortex and the hippocamps. The distal extent to which silver grains cold be visualized in the pyramidal cell dendrite was comparable to that seen with a probe to ribosomal RNA. In contrast to the tau probe the ribosomal probe also labeled glial cells. Sections hybridized with the tau probe and then double‐labeled with thioflavine S revealed that neurons containing neurofibrillary tangles tangles continue to synthesize tau protein.