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Human cytotoxic T‐cell recognition of a synthetic peptide of myelin basic protein
Author(s) -
Richert John R.,
Robinson Eve D.,
Deibler Gladys E.,
Martenson Russell E.,
Dragovic Ljubo J.,
Kies Marian W.
Publication year - 1989
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410260306
Subject(s) - cytotoxic t cell , myelin basic protein , peptide , epitope , myelin , biology , biochemistry , peptide sequence , t cell , microbiology and biotechnology , chemistry , immunology , in vitro , immune system , antigen , central nervous system , gene , neuroscience
Previous studies with a panel of myelin basic protein (MBP)–specific human T‐cell clones suggested a clustering of epitopes in the middle and at the C terminus of the molecule. The current study demonstrates that 19 of 40 clones recognize a synthetic peptide corresponding to residues 152 to 170 of the human MBP molecule and that 9 clones recognize a synthetic peptide corresponding to residues 86 to 105. Myelin basic protein–specific cytotoxic activity was restricted to the clones that recognized peptide 152–170, and this peptide served as a preferential cytotoxic T‐cell target when attached to an autologous B‐cell line. The specificity of MBP‐directed cytotoxic activity appears to be much more restricted than the specificity demonstrated for proliferative activity.