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Dual‐label immunocytochemistry of the active multiple sclerosis lesion: Major histocompatibility complex and activation antigens
Author(s) -
Hayashi T.,
Morimoto C.,
Burks J. S.,
Kerr C.,
Hauser S. L.
Publication year - 1988
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410240408
Subject(s) - major histocompatibility complex , cd1 , cd8 , biology , antigen , cytotoxic t cell , immunocytochemistry , population , monoclonal antibody , immunology , mhc class i , t cell , mhc class ii , pathology , antibody , microbiology and biotechnology , immune system , natural killer t cell , medicine , endocrinology , genetics , in vitro , environmental health
Fresh‐frozen autopsy material containing active inflammatory lesions from 9 different patients with multiple sclerosis (MS) was analyzed by immunocytochemistry using a panel of monoclonal antibodies, and a dual‐label immunocytochemical method was developed which permitted the simultaneous detection of two different surface Marchkers on a single cell. We now report the following. (1) The predominant T‐cell phenotype within MS lesions is CD2,3,8. This phenotype Marchks the suppressor‐cytotoxic subset. (2) These cells do not express the natural killer cell Marchker NKH‐1, which is present on a subset of CD8‐positive cells in peripheral blood. (3) The infiltrating cell expresses class I (HLA A, B, C), but not class II (DR and DQ), major histocompatibility complex (MHC) molecules. (4) Other T‐cell surface molecules, including the activation antigens interleukin‐2 receptor, Ta 1 , and Tll‐3, as well as the Marchker 2H4, are largely not expressed. (5) Endothelial cells express both class I and class II MHC molecules and the 4B4 molecule in both MS and control tissue. (6) Astrocytes within the vicinity of MS lesions are predominantly class II MHC—negative. These results demonstrate that the T‐cell infiltrate present in MS tissue on autopsy has a restricted phenotypic profile, but they also raise the possibility that, within this population, few activated effector cells are present.