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Reduction of monocyte 5′nucleotidase activity by γ‐interferon in multiple sclerosis and autoimmune diseases
Author(s) -
Armstrong Marilyn A.,
Shah Soraya,
Hawkins Stanley A.,
Bell Aubrey L.,
Roberts Stanley D.
Publication year - 1988
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410240104
Subject(s) - multiple sclerosis , 5' nucleotidase , interferon , monocyte , immunology , medicine , reduction (mathematics) , autoimmune disease , adenosine , antibody , mathematics , geometry
Ecto‐5′nucleotidase (5′NT) activity in the plasma membrane of peripheral blood monocytes from patients with multiple sclerosis (MS), rheumatoid arthritis (RA), myasthenia gravis (MG), motor neuron disease (MND), and from normal control subjects of similar age was determined by radioisotopic assay. The activity in unstimulated monocytes cultured for 24 and 48 hours was found to be higher than normal in 56% of patients with active relapsing MS, 29% of patients with RA, and 7% of patients in the MG/MND group. While the enzyme activity was reduced after cultivation of the cells with recombinant interferonrγ(γ‐IFN) in all of the groups studied, the percentage reduction was significantly greater in monocytes from patients with active relapsing MS who were in relapse at the time of sampling ( p = 0.03). HLA‐DR expression was monitored using immunofluorescence staining with monoclonal antibody and showed that similar numbers of monocytes in all patient and control groups expressed this antigen. Thus, while high monocyte 5′NT activity was found principally in patients with active relapsing MS and in some patients with RA, the monocytes from patients with MS were, in addition, exquisitely sensitive to stimulation withγ‐IFN.