Premium
Generation of human B‐cell hybridomas secreting monoclonal anti–myelin‐associated glycoprotein antibodies from a patient with neuropathy
Author(s) -
Spatz Linda,
Lieberson Becky,
Abraham Judith,
Miller Orlando J.,
Miller Dorothy A.,
Latov Norman
Publication year - 1987
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410210217
Subject(s) - idiotype , monoclonal antibody , autoantibody , myelin associated glycoprotein , antibody , monoclonal , b cell , microbiology and biotechnology , immunology , antigen , glycoprotein , myelin , cell fusion , immunoglobulin m , plasma cell , biology , secretion , cell , immunoglobulin g , endocrinology , biochemistry , central nervous system
Human hybridomas that secrete monoclonal IgM anti–myelin‐associated glycoprotein antibodies were generated by fusion with cells from a patient with peripheral neuropathy and IgM monoclonal gammopathy. Karyotypic analysis of the hybridoma cells revealed no chromosomal abnormalities. The cells were positive for cell‐surface idiotype HLA‐DR and the plasma cell antigen PCA‐1, and negative for the B‐cell determinant B4 and for Leu‐1, which has been postulated to distinguish a subpopulation of B cells that secrete IgM with autoantibody activity.