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Isolation of P 2 protein–reactive T‐cell lines from human blood
Author(s) -
Burns James,
Krasner L. Jill,
Rostami Abdolmohammad,
Pleasure David
Publication year - 1986
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410190416
Subject(s) - myelin basic protein , myelin , antigen , immunology , t cell , in vitro , biology , cell culture , multiple sclerosis , microbiology and biotechnology , central nervous system , endocrinology , immune system , biochemistry , genetics
Human T‐cell lines reactive with the peripheral nerve myelin, protein, P 2 protein, were isolated from the peripheral blood of 4 normal persons and 1 patient with Guillain‐Barré syndrome. These predominantly helper phenotype T‐cell lines were isolated and maintained in vitro by antigen stimulation followed by culture with interleukin 2. Myelin basic protein–reactive T cells were also isolated in parallel from the same subjects as antigen specificity controls. T cells recognizing myelin basic protein did not respond to P 2 protein, nor did P 2 ‐reactive cells respond to myelin basic protein. These findings suggest that a potential for autoimmune reactivity with peripheral nervous system myelin antigens may exist for both normal persons and some patients with neurological disease.