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Monoclonal IgM in a patient with paraproteinemic polyneuropathy binds to gangliosides containing disialosyl groups
Author(s) -
Ilyas Amjad A.,
Quarles Richard H.,
Dalakas Marinos C.,
Fishman Peter H.,
Brady Roscoe O.
Publication year - 1985
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410180605
Subject(s) - paraproteinemia , ganglioside , immunoglobulin m , monoclonal , paraproteins , polyneuropathy , chemistry , monoclonal antibody , paraproteinemias , antibody , immunology , microbiology and biotechnology , medicine , immunoglobulin g , pathology , biochemistry , biology , multiple myeloma
Abstract Monoclonal IgM K from a patient with polyneuropathy associated with paraproteinemia was found to bind to several polysialogangliosides. Binding of IgM to gangliosides was shown by enzyme‐linked immunosorbent assay (ELISA) and by overlaying thin‐layer chromatograms of human brain and peripheral nerve gangliosides with the patient's serum followed by radioiodinated goat antihuman IgM. The latter technique showed that the IgM paraprotein reacted with a number of gangliosides. In an ELISA the IgM paraprotein reacted strongly with GD 2 , GD 3 , GD 1b , and GT 1b , but not with GM 1 , GM 3 , and GD 1a . Thus, the epitope for the patient's IgM paraprotein appears to involve the disialosyl configurations.