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Progressive multifocal leukoencephalopathy: Investigation of three cases using in situ hybridization with JC virus biotinylated DNA probe
Author(s) -
Aksamit Allen J.,
Mourrain Pascale,
Sever John L,
Major Eugene O.
Publication year - 1985
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410180412
Subject(s) - jc virus , progressive multifocal leukoencephalopathy , in situ hybridization , biology , virus , biotinylation , viral replication , virology , dna , hybridization probe , slow virus , microbiology and biotechnology , bk virus , nucleic acid thermodynamics , pathology , medicine , gene , kidney , genetics , gene expression , kidney transplantation , base sequence
Using the technique of in situ DNA‐to‐DNA hybridization, a JC virus biotinylated DNA probe was developed and applied to formalin‐fixed, paraffin‐embedded, or fixed, frozen sections of brain tissue from three subjects with progressive multifocal leukoencephalopathy (PML). Light microscopy was carried out to correlate the presence of JC virus DNA with the selective infection of oligodendrocytes and astrocytes in PML. Oligodendrocytes (lytically infected) showed the greatest evidence of viral DNA. More astrocytes showing bizarre morphological changes had evidence of viral DNA than did astrocytes that were simply reactive. Viral DNA was not evident in vascular endothelial cells using this technique. Viral DNA replication may be an important initial step which produces the bizarre “transformed” astrocytes of PML. Findings in this study do not support the hypothesis that vascular endothelial replication is important in the pathogenesis of JC virus–induced PML. In situ hybridization with biotinylated JC virus probe may be useful in the diagnosis of PML on brain biopsy specimens.