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Detection of picornavirus sequences in nervous tissue of amyotrophic lateral sclerosis and control patients
Author(s) -
Brahic M.,
Smith R. A.,
Gibbs C. J.,
Garruto R. M.,
Tourtellotte W. W.,
Cash E.
Publication year - 1985
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410180311
Subject(s) - amyotrophic lateral sclerosis , picornavirus , medicine , multiple sclerosis , physical medicine and rehabilitation , nervous system , neuroscience , biology , pathology , disease , immunology , genetics , rna , gene
We used in situ hybridization to look for picornavirus ribonucleic acid (RNA) sequences in frozen sections of central nervous system (CNS) tissue of amyotrophic lateral sclerosis (ALS) and control patients. Using reconstruction experiments, we concluded that 30 copies of viral RNA per cell could be detected with the assay. RNA which hybridized to DNAs complementary (cDNAs) to both poliovirus and Theiler's virus was found at several levels in the CNS of 2 patients, 1 ALS patient, and 1 control. In transverse sections of the spinal cord, these sequences predominated in cells of the anterior horns. We assessed the specificity of hybridization by several criteria: (1) no hybridization was observed with heterologous visna virus cDNA probes; (2) hybridization was abolished by pretreatment of the sections with ribonuclease; (3) chemography artifacts were ruled out; and (4) the results were reproduced in three independent experiments. We concluded that RNA molecules, possibly belonging to a picornavirus having sequences in common with poliovirus and Theiler's virus, were present in the CNS of these 2 patients. On the other hand, 14 cases of classic ALS, 2 cases of Guamanian parkinsonian dementia, and 5 controls had negative results. However, the presence of picornavirus sequences in our series could be underestimated because in many cases autolysis times were 10 hours or longer.