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Stability of monoamine metabolites in human cerebrospinal fluid
Author(s) -
Langlais Philip J.,
Bird Edward D.,
McEntee William J.
Publication year - 1982
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410120109
Subject(s) - homovanillic acid , monoamine neurotransmitter , metabolite , cerebrospinal fluid , ascorbic acid , chemistry , chromatography , lumbar puncture , dopamine , medicine , biochemistry , food science , serotonin , receptor
Concentrations of 3‐methoxy‐4‐hydroxyphenylglycol (MHPG), homovanillic acid (HVA), and 5‐hydroxyindoleacetic acid (5‐HIAA) were measured in human lumbar crerospinal fluid following storage at room temperature and 4°C for intervals up to 72 hours. These monoamine metabolites were quantitated using a high‐performance liquid chromatographic—electrochemical detector technique. No significant difference could be detected in any of the three metabolites stored at room temperature for up to 48 hours. Additionof ascorbic acid to CSF had no measurable effect on the levels of HVA and 5‐HIAA. Repeated thawing and freezing produced no detectable change in metabolite levels. The results indicate that these monoamine metabolites are stable at room temperature for up to 48 hours after lumbar puncture and that the addition of an antioxdant, such as ascorbic acid, is not necessary. The data also demonstrate that immediate refrigeration or freezing of samples is not required for maintenance of metabolite levels.