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Myotonic dystrophy: Calcium‐dependent phosphatidic acid synthesis in erythrocytes
Author(s) -
Moore R. B.,
Appel S. H.,
Plishker G. A.
Publication year - 1981
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410100515
Subject(s) - phosphatidic acid , myotonic dystrophy , calcium , chemistry , biochemistry , inorganic phosphate , muscular dystrophy , medicine , endocrinology , phosphate , phospholipid , biology , organic chemistry , membrane
Recently it was reported that calcium‐dependent phosphatidic acid synthesis in erythrocytes of patients with myotonic muscular dystrophy (MyD) is markedly impaired when compared to that in control subjects. Using 32 P‐loaded erythrocytes, we found no significant difference in the levels of 32 P‐phosphatidic acid synthesized after exposure to calcium and its ionophore A23187 between patients with MyD and controls. In a batch experiment typical of the experiments with 32 P, a twofold increase of phosphatidic acid in both groups was determined by inorganic phosphate measurements. Thus, the specific activity of the 32 P‐phosphatidic acid increased four‐ to five‐fold in response to calcium. Analyses of 32 P‐polyphosphoinositide breakdown in ghosts and in adenosine triphosphate‐depleted erythrocytes also appeared normal for patients with myotonic muscular dystrophy. Possible discrepancies between the results presented here and those reported previously are discussed.