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Tomographic measurement of local cerebral glucose metabolic rate in humans with (F‐18)2‐fluoro‐2‐deoxy‐D‐glucose: Validation of method
Author(s) -
Phelps M. E.,
Huang S. C.,
Hoffman E. J.,
Selin C.,
Sokoloff L.,
Kuhl D. E.
Publication year - 1979
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.410060502
Subject(s) - positron emission tomography , chemistry , reproducibility , nuclear medicine , venous blood , tracer , metabolic activity , metabolic rate , positron , chromatography , medicine , physics , physiology , quantum mechanics , nuclear physics , electron
Tracer techniques and quantitative autoradiographic and tissue counting models for measuremnt of metabolic rates were combined with positron computed tomography (PCT) and (F‐18)2‐fluoro‐2‐deoxy‐D‐glucose (FDG) for the measurement of local cerebral metabolic rate for glucose (LCMRGlc) in humans. A three‐compartment model, which incorporates hydrolysis of FDG‐6‐PO 4 to FDG, was developed for the measure of kinetic constants and calculation of LCMRGlc. Our model is an extension of that developed by Sokoloff et al. Although small, hydrolysis of FDG‐6‐PO 4 was found to be significant. A PCT system, the ECAT, was used to determine the rate constants, lumped constant, and stability of the model in human beings. The data indicate that cerebral FDG‐6‐PO 4 in humans increases for about 90 minutes, plateaus, and then slowly decreases. After 10 minutes, cerebral blood FDG activity levels were found to be a minor fraction of tissue activity. Precursor pool turnover rate, distribution volumes, and red blood cell‐plasma concentration ratios were determined. Reproducibility (precision) of LCMRGlc measurements (∼2 cm 2 regions) was ± 5.5% over a 5‐hour period. The replacement of arterial blood sampling with venous sampling was validated.