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Human Metabotropic Glutamate Receptor 5 Antibodies Alter Receptor Levels and Behavior in Mice
Author(s) -
Maudes Estibaliz,
Mannara Francesco,
GarcíaSerra Anna,
Radosevic Marija,
Mellado Araceli,
Serafim Ana Beatriz,
Planagumà Jesús,
Sabater Lidia,
Dalmau Josep,
Spatola Marianna
Publication year - 2022
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.26362
Subject(s) - metabotropic glutamate receptor 5 , hippocampal formation , metabotropic glutamate receptor , glutamate receptor , antibody , hippocampus , encephalitis , metabotropic glutamate receptor 4 , receptor , medicine , neuroscience , biology , endocrinology , immunology , virus
Ophelia syndrome or encephalitis with antibodies against the metabotropic glutamate receptor 5 (mGluR5) manifests with behavioral changes, memory deficits, and anxiety. To study the antibody pathogenicity, mice received continuous cerebroventricular infusion of patients' or controls' immunoglobulin G (IgG) for 14 days, followed by a 15‐day washout. The effects on hippocampal mGluR5 clusters were determined by confocal microscopy. Animals infused with patients' IgG, but not controls' IgG, showed memory impairment, increased anxiety, and decreased neuronal surface mGluR5 clusters. After antibody clearance, both behavioral and molecular changes reversed to baseline conditions. These findings support the pathogenicity of these antibodies in anti‐mGluR5 encephalitis. ANN NEUROL 2022;92:81–86