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Haploinsufficiency of Cyfip2 Causes Lithium‐Responsive Prefrontal Dysfunction
Author(s) -
Lee SeungHyun,
Zhang Yinhua,
Park Jina,
Kim Bowon,
Kim Yangsik,
Lee Sang Hoon,
Kim Gyu Hyun,
Huh Yang Hoon,
Lee Bokyoung,
Kim Yoonhee,
Lee Yeunkum,
Kim Jin Yong,
Kang Hyojin,
Choi SuYeon,
Jang Seil,
Li Yan,
Kim Shinhyun,
Jin Chunmei,
Pang Kaifang,
Kim Eunjeong,
Lee Yoontae,
Kim Hyun,
Kim Eunjoon,
Choi Jee Hyun,
Kim Jeongjin,
Lee Kea Joo,
Choi SeYoung,
Han Kihoon
Publication year - 2020
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.25827
Subject(s) - prefrontal cortex , dendritic spine , neuroscience , psychology , hippocampal formation , cognition
Objective Genetic variants of the cytoplasmic FMR1‐interacting protein 2 ( CYFIP2 ) encoding an actin‐regulatory protein are associated with brain disorders, including intellectual disability and epilepsy. However, specific in vivo neuronal defects and potential treatments for CYFIP2 ‐associated brain disorders remain largely unknown. Here, we characterized Cyfip2 heterozygous ( Cyfip2 +/− ) mice to understand their neurobehavioral phenotypes and the underlying pathological mechanisms. Furthermore, we examined a potential treatment for such phenotypes of the Cyfip2 +/− mice and specified a neuronal function mediating its efficacy. Methods We performed behavioral analyses of Cyfip2 +/− mice. We combined molecular, ultrastructural, and in vitro and in vivo electrophysiological analyses of Cyfip2 +/− prefrontal neurons. We also selectively reduced CYFIP2 in the prefrontal cortex (PFC) of mice with virus injections. Results Adult Cyfip2 +/− mice exhibited lithium‐responsive abnormal behaviors. We found increased filamentous actin, enlarged dendritic spines, and enhanced excitatory synaptic transmission and excitability in the adult Cyfip2 +/− PFC that was restricted to layer 5 (L5) neurons. Consistently, adult Cyfip2 +/− mice showed increased seizure susceptibility and auditory steady‐state responses from the cortical electroencephalographic recordings. Among the identified prefrontal defects, lithium selectively normalized the hyperexcitability of Cyfip2 +/− L5 neurons. RNA sequencing revealed reduced expression of potassium channel genes in the adult Cyfip2 +/− PFC. Virus‐mediated reduction of CYFIP2 in the PFC was sufficient to induce L5 hyperexcitability and lithium‐responsive abnormal behavior. Interpretation These results suggest that L5‐specific prefrontal dysfunction, especially hyperexcitability, underlies both the pathophysiology and the lithium‐mediated amelioration of neurobehavioral phenotypes in adult Cyfip2 +/− mice, which can be implicated in CYFIP2 ‐associated brain disorders. ANN NEUROL 2020;88:526–543