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Maternal immune activation promotes hippocampal kindling epileptogenesis in mice
Author(s) -
Pineda Eduardo,
Shin Don,
You Su Jeong,
Auvin Stéphane,
Sankar Raman,
Mazarati Andréy
Publication year - 2013
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.23898
Subject(s) - kindling , epileptogenesis , offspring , epilepsy , hippocampal formation , cytokine , immune system , immunology , endocrinology , medicine , psychology , neuroscience , biology , pregnancy , genetics
Objective Maternal immune activation (MIA) triggered by infections has been identified as a cause of autism in offspring. Considering the involvement of perturbations in innate immunity in epilepsy, we examined whether MIA represents a risk factor for epilepsy as well. The role of specific MIA components interleukin (IL)‐6 and IL‐1β was also addressed. Methods MIA was induced in C57BL/6 mice by polyinosinic–polycytidylic acid (PIC) injected during embryonic days 12 to 16. Beginning from postnatal day 40, the propensity of the offspring to epilepsy was examined using hippocampal kindling; autismlike behavior was studied using the sociability test. The involvement of IL‐6 and IL‐1β in PIC‐induced effects was studied by the coadministration of the cytokine antibodies with PIC, and by delivering recombinant cytokines in lieu of PIC. Results The offspring of PIC‐exposed mice exhibited increased hippocampal excitability, accelerated kindling rate, prolonged increase of seizure susceptibility after kindling, and diminished sociability. Epileptic impairments were abolished by antibodies to IL‐6 or IL‐1β. Neither of the recombinant cytokines alone increased the propensity to seizures; however, when combined, they produced effects similar to those induced by PIC. PIC‐induced behavioral deficits were abolished by IL‐6 antibodies and were mimicked by recombinant IL‐6; IL‐1β was not involved. Interpretation In addition to confirming the previously established critical role of IL‐6 in the development of autismlike behavior following MIA, the present study shows that concurrent involvement of IL‐6 and IL‐1β is required for priming the offspring for epilepsy. These data shed light on mechanisms of comorbidity between autism and epilepsy. Ann Neurol 2013;74:11–19