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Cerebrospinal fluid of brain trauma patients inhibits in vitro neuronal network function via NMDA receptors
Author(s) -
Otto Frauke,
Illes Sebastian,
Opatz Jessica,
Laryea Maurice,
Theiss Stephan,
Hartung HansPeter,
Schnitzler Alfons,
Siebler Mario,
Dihné Marcel
Publication year - 2009
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.21808
Subject(s) - cerebrospinal fluid , receptor , in vitro , nmda receptor , glasgow coma scale , neuroscience , glutamate receptor , traumatic brain injury , medicine , premovement neuronal activity , chemistry , pathology , biology , anesthesia , biochemistry , psychiatry
Neurological diseases frequently induce pathological changes of cerebrospinal fluid (CSF) that might secondarily influence brain activity, as the CSF–brain barrier is partially permeable. However, functional effects of CSF on neuronal network activity have not been specified to date. Here, we report that CSF specimens from patients with reduced Glasgow Coma Scale values caused by severe traumatic brain injury suppress synchronous activity of in vitro‐generated neuronal networks in comparison with controls. We present evidence that underlying mechanisms include increased N ‐methyl‐ D‐ aspartate receptor activity mediated by a CSF fraction containing elevated amino acid concentrations. These proof‐of‐principle data suggest that determining effects of CSF specimens on neuronal network activity might be of diagnostic value. Ann Neurol 2009;66:546–555