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Autoantibodies in gluten ataxia recognize a novel neuronal transglutaminase
Author(s) -
Hadjivassiliou Marios,
Aeschlimann Pascale,
Strigun Alexander,
Sanders David S.,
Woodroofe Nicola,
Aeschlimann Daniel
Publication year - 2008
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.21450
Subject(s) - tissue transglutaminase , autoantibody , immunology , ataxia , antibody , gluten , autoimmunity , immune system , medicine , enteropathy , biology , disease , pathology , enzyme , biochemistry , psychiatry
Objective Gluten sensitivity typically presents as celiac disease, a chronic, autoimmune‐mediated, small‐intestinal disorder. Neurological disorders occur with a frequency of up to 10% in these patients. However, neurological dysfunction can also be the sole presenting feature of gluten sensitivity. Development of autoimmunity directed toward different members of the transglutaminase gene family could offer an explanation for the diversity in manifestations of gluten sensitivity. We have identified a novel neuronal transglutaminase isozyme and investigated whether this enzyme is the target of the immune response in patients with neurological dysfunction. Methods Using recombinant human transglutaminases, we developed enzyme‐linked immunosorbent assays and inhibition assays to analyze serum samples of patients with gluten‐sensitive gastrointestinal and neurological disorders, and various control groups including unrelated inherited or immune conditions for the presence and specificity of autoantibodies. Results Whereas the development of anti‐transglutaminase 2 IgA is linked with gastrointestinal disease, an anti‐transglutaminase 6 IgG and IgA response is prevalent in gluten ataxia, independent of intestinal involvement. Such antibodies are absent in ataxia of defined genetic origin or in healthy individuals. Inhibition studies showed that in those patients with ataxia and enteropathy, separate antibody populations react with the two different transglutaminase isozymes. Furthermore, postmortem analysis of brain tissue showed cerebellar IgA deposits that contained transglutaminase 6. Interpretation Antibodies against transglutaminase 6 can serve as a marker in addition to human leukocyte antigen type and detection of anti‐gliadin and anti‐transglutaminase 2 antibodies to identify a subgroup of patients with gluten sensitivity who may be at risk for development of neurological disease. Ann Neurol 2008;64:332–343