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Amyloid‐β oligomers are inefficiently measured by enzyme‐linked immunosorbent assay
Author(s) -
Stenh Charlotte,
Englund Hillevi,
Lord Anna,
Johansson AnnSofi,
Almeida Claudia G.,
Gellerfors Pär,
Greengard Paul,
Gouras Gunnar K.,
Lannfelt Lars,
Nilsson Lars N. G.
Publication year - 2005
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.20524
Subject(s) - enzyme , amyloid β , amyloid (mycology) , peptide , biochemistry , alzheimer's disease , amyloid beta , chemistry , disease , microbiology and biotechnology , biology , medicine , inorganic chemistry
Amyloid‐β (Aβ) peptide levels are widely measured by enzyme‐linked immunosorbent assay (ELISA) in Alzheimer's disease research. Here, we show that oligomerization of Aβ results in underestimated Aβ ELISA levels. The implications are that comprehensive analysis of soluble Aβ requires either sample pretreatment at denaturing conditions or novel conformation‐dependent immunoassays. Our findings might be of relevance for many neurodegenerative disorders in which soluble protein aggregates are the main neurotoxic species. Ann Neurol 2005;58:147–150