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Gene transfer of glutamic acid decarboxylase reduces neuropathic pain
Author(s) -
Hao Shuanglin,
Mata Marina,
Wolfe Darren,
Glorioso Joseph C.,
Fink David J.
Publication year - 2005
Publication title -
annals of neurology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 4.764
H-Index - 296
eISSN - 1531-8249
pISSN - 0364-5134
DOI - 10.1002/ana.20483
Subject(s) - glutamate decarboxylase , dorsal root ganglion , nociception , spinal cord , herpes simplex virus , neuropathic pain , chemistry , pharmacology , biology , neuroscience , immunology , biochemistry , virus , enzyme , receptor
Abstract We tested whether transfer of the gene coding for glutamic acid decarboxylase to dorsal root ganglion using a herpes simplex virus vector to achieve release of GABA in dorsal horn would attenuate nociception in this condition. Subcutaneous inoculation of a replication‐defective herpes simplex virus vector expressing glutamic acid decarboxylase (vector QHGAD67) 7 days after selective L5 spinal nerve ligation reversed mechanical allodynia and thermal hyperalgesia; the antiallodynic effect lasted 6 weeks and was reestablished by reinoculation. QHGAD67 inoculation also suppressed induction of c‐Fos and phosphorylated extracellular signal–regulated kinase 1 and 2 in the spinal cord. Ann Neurol 2005;57:914–918