Open AccessGenomic analysis of antibiotic resistance for Acinetobacter baumannii in a critical care center
Author(s) -
Murata Kensuke,
Inoue Yoshiaki,
Kaiho Mayuko,
Nakazawa Takeshi,
Sasaki Shinichi,
Miyake Kazunori,
Matsuda Shigeru,
Tanaka Hiroshi
Publication year - 2019
Publication title -
acute medicine and surgery
Language(s) - English
Resource type - Journals
ISSN - 2052-8817
DOI - 10.1002/ams2.445
Subject(s) - acinetobacter baumannii , multilocus sequence typing , antibiotic resistance , genotype , microbiology and biotechnology , biology , antibiotics , drug resistance , polymerase chain reaction , molecular epidemiology , pulsed field gel electrophoresis , typing , genetics , gene , pseudomonas aeruginosa , bacteria
Aim Acinetobacter baumannii is commonly associated with outbreaks and antibiotic‐resistant nosocomial infection. This study aimed to determine the relationship between antibiotic resistance and genotypes of A. baumannii . Methods A study was undertaken in the critical care center ( CCC ) of Juntendo University Urayasu Hospital (Urayasu, Japan) between January 2012 and September 2015. Antimicrobial susceptibility tests were carried out according to the Clinical and Laboratory Standards Institute guidelines. All A. baumannii isolates were verified to carry carbapenemase genes and the IS A ba1 element using polymerase chain reaction. The genetic relationship of all A. baumannii isolates was determined by pulsed‐field gel electrophoresis and multilocus sequence typing. Results During the study period, 1634 patients were admitted to the CCC . Acinetobacter baumannii was detected in 43 patients (average age, 58 ± 19 years; 67.4% men). Six patients were determined to be extensively drug‐resistant A. baumannii and 21 patients determined to be multidrug‐resistant A. baumannii . Antimicrobial susceptibility linked genotypes of A. baumannii . Molecular characterization by pulsed‐field gel electrophoresis and multilocus sequence typing showed that closely related clones of A. baumannii had spread in the CCC . Conclusion Resistance to antimicrobial drugs was significantly associated with certain A. baumannii genotypic types and molecular types. Thus, we might be able to predict whether the genotype has spread in the CCC or not when the susceptibility is examined, facilitating the appropriate isolation of patients.