Open AccessRefining murine heterotopic heart transplantation: A model to study ischemia and reperfusion injury in donation after circulatory death hearts
Author(s) -
Quader Mohammed,
Cholyway Renee,
Wickramaratne Niluka,
Akande Oluwatoyin,
Mangino Martin,
Mezzaroma Eleonora,
Mauro Adolfo G.,
Chen Qun,
Kantlis Alexander,
Toldo Stefano
Publication year - 2021
Publication title -
animal models and experimental medicine
Language(s) - English
Resource type - Journals
ISSN - 2576-2095
DOI - 10.1002/ame2.12176
Subject(s) - medicine , heart transplantation , transplantation , organ donation , ischemia , donation , animal model , reperfusion injury , intensive care medicine , circulatory system , cardiology , surgery , economics , economic growth
Heart transplantation is a lifesaving procedure, which is limited by the availability of donor hearts. Using hearts from donors after circulatory death, which have sustained global ischemia, requires thorough studies on reliable and reproducible models that developing researchers may not have mastered. By combining the most recent literature and our recommendations based on observations and trials and errors, the methods here detail a sound in vivo heterotopic heart transplantation model for rats in which protective interventions on the ischemic heart can be studied, and thus allowing the scientific community to advance organ preservation research. Knowledge gathered from reproducible animal models allow for successful translation to clinical studies.