Open AccessAn in vivo study of the pharmacological activities of a methanolic acetate fraction of Pistia stratiotes L.: An ethno‐medicinal plant used in Bangladesh
Author(s) -
Hussain Md. Saddam,
Ibrahim Md.,
Hasan Md. Murad,
Aziz Mohammad Tarek,
Suchi Suzia Akter,
Uddin Md. Giash
Publication year - 2018
Publication title -
animal models and experimental medicine
Language(s) - English
Resource type - Journals
ISSN - 2576-2095
DOI - 10.1002/ame2.12037
Subject(s) - licking , pistia , stratiotes , carrageenan , depressant , analgesic , traditional medicine , open field , pharmacology , anti inflammatory , acetic acid , in vivo , chemistry , medicine , biology , biochemistry , microbiology and biotechnology , ecology , macrophyte , aquatic plant
Background The present study covered an assessment of methanolic acetate fractions of Pistia stratiotes , commonly known as water lettuce, for analgesic, anti‐inflammatory, and CNS depressant activities. Methods The methanol acetate fractions were extracted from Pistia stratiotes and administered to the experimental animals as 200 and 400 mg/kg doses to determine the effect on acetic acid‐induced writhing and formalin‐induced licking and biting, for the assessment of analgesic activity. The anti‐inflammatory assay was done using the carrageenan‐induced hind paw edema method, while assessment of CNS depressant activity was done using the open field and hole cross tests. The effects were compared to standard reference drugs. Results At both doses (200 and 400 mg/kg body weight), the methanolic acetate extract of P. stratiotes showed significant analgesic action ( P < 0.05) against acetic acid‐induced writhing. The extract was also found to give significant protection against licking and biting at both doses. The methanolic acetate extract of P. stratiotes showed a significant ( P < 0.05) anti‐inflammatory effect from 0 minutes up to 3 hours in the carrageenan‐induced paw edema test. In the CNS depressant assay, the methanolic acetate extract showed significant ( P < 0.05) depressant activity at both doses from 30 to 120 minutes in both the hole cross and open field tests. Conclusion Thus we can conclude that P. stratiotes extracts have significant analgesic, anti‐inflammatory, and CNS depressant activity, compared to standard compounds, in an animal model.