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Characterization of pre‐analytical sample handling effects on a panel of Alzheimer's disease–related blood‐based biomarkers: Results from the Standardization of Alzheimer's Blood Biomarkers (SABB) working group
Author(s) -
Verberk Inge M. W.,
Misdorp Els O.,
Koelewijn Jannet,
Ball Andrew J.,
Blennow Kaj,
Dage Jeffrey L.,
Fandos Noelia,
Hansson Oskar,
Hirtz Christophe,
Janelidze Shorena,
Kang Sungmin,
Kirmess Kristopher,
Kindermans Jana,
Lee Ryan,
Meyer Matthew R.,
Shan Dandan,
Shaw Leslie M.,
Waligorska Teresa,
West Tim,
Zetterberg Henrik,
Edelmayer Rebecca M.,
Teunissen Charlotte E.
Publication year - 2022
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1002/alz.12510
Subject(s) - centrifugation , blood collection , whole blood , alzheimer's disease , ethylene diamine , glial fibrillary acidic protein , medicine , chemistry , chromatography , pathology , immunology , disease , emergency medicine , immunohistochemistry , nuclear chemistry
Pre‐analytical sample handling might affect the results of Alzheimer's disease blood‐based biomarkers. We empirically tested variations of common blood collection and handling procedures. Methods We created sample sets that address the effect of blood collection tube type, and of ethylene diamine tetraacetic acid plasma delayed centrifugation, centrifugation temperature, aliquot volume, delayed storage, and freeze–thawing. We measured amyloid beta (Aβ)42 and 40 peptides with six assays, and Aβ oligomerization‐tendency (OAβ), amyloid precursor protein (APP) 699‐711 , glial fibrillary acidic protein (GFAP), neurofilament light (NfL), total tau (t‐tau), and phosphorylated tau181. Results Collection tube type resulted in different values of all assessed markers. Delayed plasma centrifugation and storage affected Aβ and t‐tau; t‐tau was additionally affected by centrifugation temperature. The other markers were resistant to handling variations. Discussion We constructed a standardized operating procedure for plasma handling, to facilitate introduction of blood‐based biomarkers into the research and clinical settings.