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Human Herpesvirus detection and identification in postmortem brain tissue of patients with Alzheimer's disease
Author(s) -
Gomez Johana,
VillegasLanau Carlos Andrés,
Mesa Ana
Publication year - 2021
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1002/alz.058693
Subject(s) - disease , multiplex , dementia , etiology , multiplex polymerase chain reaction , biology , microarray , pathogenesis , identification (biology) , human herpesvirus 6 , alzheimer's disease , microbiome , pathology , virology , medicine , immunology , herpesviridae , virus , polymerase chain reaction , bioinformatics , gene , viral disease , genetics , gene expression , botany
Background The etiology of Alzheimer’s disease (AD) is not clear yet. One of the hypotheses in this regard is the participation of infectious agents in the origin of this neurodegenerative disease. Different viruses, mainly those that cause latent infections such as some of the Herpesviridae family, have been the most involved. The aim of this study was to detect and identify herpesviruses in postmortem brain tissue of patients with AD. Method frozen brain tissues from patients diagnosed with AD (n=22) and from healthy individuals (n=10), as a control group, were included. The search and identification of Human Herpesvirus in the tissues was carried out using microarray technology (Axiom™ Microbiome Array, Thermo scientific, USA) and real‐time multiplex PCR. Result no viruses were detected using the microarray technology; howeverer, using real‐time multiplex PCR, human herpesviruses VHH‐1, VHH‐2 or VHH4 were identified in two samples of patients with AD and one case in the control group. Conclusion Based on the results obtained in this study, it was not possible to establish an association between AD and the presence of viruses. Yet, it is possible that the viruses play a role in the pathogenesis of AD, perhaps in the early stages of this dementia.