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Molecular alterations of protein tau in the cingulate cortex of human brain with Alzheimer’s disease
Author(s) -
ApatigaPerez Ricardo,
De la Cruz Fidel,
GarcésRamírez Linda,
HernandesAlejandro Mario,
OntiverosTorres Miguel Ángel,
LunaMuñoz José
Publication year - 2020
Publication title -
alzheimer's and dementia
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 6.713
H-Index - 118
eISSN - 1552-5279
pISSN - 1552-5260
DOI - 10.1002/alz.041127
Subject(s) - hippocampus , cingulate cortex , cortex (anatomy) , posterior cingulate , alzheimer's disease , entorhinal cortex , anterior cingulate cortex , cerebral cortex , neuroscience , tau protein , temporal cortex , pathology , psychology , medicine , central nervous system , disease , cognition
Abstract Background Alzheimer's disease (AD) brain tissue at different stages were examined to compare the events of protein tau hiperphosphorilation and its assemble into Neurofibrillary tangles (NFT) in the hippocampus and the cingulate cortex and how this may be involved in the appearance of the Behavioral and Psychological Symptoms of Dementia (BPSD). Method Brain tissue previously fixed with 4% paraformaldehyde from 7 patients with different stages of AD were examined in this study. Sections were blocked with 0.2% IgG free albumin and the primary antibody (Table 1) was incubated overnight. Secondary antibody HRP was incubated considering the specie and isotype. Finally, the reaction was revealed with 0.0006% 3´3´diaminobenzidine and 0.006% H 2 O 2Result The cases with early stages of AD are morphologically characterized by diffuse and granular deposits of tau aggregate in a non‐fibrillar state in the transentorhinal region of the hippocampus and to a lesser extent in the cingulate cortex. Following this pattern, in cases with mild stage was found in less proportion extracellular NFT (E‐NFT) in CA1 region of the hippocampus, in contrast, there was a major labelling for this antibody in the cingulate cortex but for intracellular NFT. Finally, in moderate AD cases was found E‐NFT both in the cingulate cortex and hippocampus, being the latter where it was observed to a greater extent. Conclusion There is a direct relationship in the processes of NFT formation and subsequent disease progression between the cingulate cortex and the hippocampus, having its origin in the latter.