Premium
Olfactory cleft mucus proteome in chronic rhinosinusitis: a case‐control pilot study
Author(s) -
Soler Zachary M.,
Schlosser Rodney J.,
Mulligan Jennifer K.,
Smith Timothy L.,
Mace Jess C.,
Ramakrishan Vijay R.,
NorrisCaneda Kim,
Bethard Jennifer R.,
Ball Lauren E.
Publication year - 2021
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.22743
Subject(s) - proteome , mucus , medicine , fold change , chronic rhinosinusitis , olfactory mucosa , odorant binding protein , olfaction , proteomics , biochemistry , olfactory system , immunology , biology , neuroscience , ecology , downregulation and upregulation , psychiatry , gene
Background Mechanisms of smell loss in chronic rhinosinusitis (CRS) are still unclear and likely multifactorial. Little attention has been given to olfactory cleft (OC) mucus proteins involved in odorant binding and metabolizing enzymes and their potential role in smell loss. Methods Mucus from the OC was sampled from patients with CRS (n = 20) and controls (n = 10). Liquid chromatography and mass spectrometry were performed, followed by data processing so that protein groups could be identified, quantified, and compared. Hierarchical clustering and bioinformatic analysis were performed on significantly different proteins to explore for enrichment in known biologic pathways. Results A total of 2514 proteins were found in OC mucus from all 30 subjects. Significant differences in protein abundance were found between CRS and controls, including both CRSsNP (n = 351 proteins; log 2 fold change range: –3.88 to 6.71) and CRSwNP (n = 298 proteins; log 2 fold change range: –4.00 to –6.13). Significant differences were found between patients with normosmia and those with dysosmia (n = 183; log 2 fold change range: –3.62 to –2.16) and across groups of interest for a number of odorant binding proteins and metabolizing enzymes. Conclusion OC mucous in CRS displays a rich and abundant array of proteins, many of which have been implicated in odorant transport and metabolization in animal studies. Significant differences in the olfactory mucus proteome were seen between CRS subtypes and controls, as well as between those with normal and abnormal olfaction. Further study should confirm these findings and explore the role individual proteins play in odorant transport and metabolization. ©2020 ARSAAOA, LLC.