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Infiltration pattern of gammadelta T cells and its association with local inflammatory response in the nasal mucosa of patients with allergic rhinitis
Author(s) -
Yang Qintai,
Li Chunwei,
Wang Weihao,
Zheng Rui,
Huang Xuekun,
Deng Huiyi,
Jin Peng,
Tan Kaisen,
Yan Yan,
Wang Deyun
Publication year - 2019
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.22421
Subject(s) - immunology , foxp3 , immune system , medicine , chemokine , innate lymphoid cell , cd8 , inflammation , t cell , mucous membrane of nose , infiltration (hvac) , rar related orphan receptor gamma , interleukin 21 , acquired immune system , physics , thermodynamics
Background gammadelta (γδ) T cells play important roles in allergic lower airway inflammation. However, little is known about their infiltration pattern in the nasal mucosa during upper airway inflammation. This study investigated γδ T cell distribution in nasal tissues of allergic rhinitis (AR) patients and the relationship between γδ T cells and other inflammatory cell types. Methods A total of 30 patients with septal deviation were examined, including 22 with and 8 without AR. The localization of γδ T cells and other cells (eosinophils, neutrophils, mast cells, macrophages, B cells, cluster of differentiation [CD]4+ T cells, CD8+ T cells, regulatory T cells [Tregs], interferon [IFN]‐γ+ cells, interleukin [IL]17+ cells, and IL10+ cells) was evaluated by histological analysis and immunohistochemistry. T helper cell (Th)1/Th2/Th17 and Treg gene expression was analyzed by quantitative polymerase chain reaction (PCR). Results γδ T cells were localized in the epithelium or subepithelial region of nasal mucosa, and their infiltration was higher in AR patients relative to control subjects. The number of γδ T cells was associated with the presence of eosinophils, macrophages, mast cells, B cells, CD8+ T cells, Forkhead box (Fox)p3+ Tregs, IL17+ cells, and IL10+ cells but not of neutrophils or IFN‐γ+ cells. The messenger RNA (mRNA) level of a γδ T cell subunit was positively correlated with those of Th1 genes (T‐bet and IFN‐γ), Th2 cytokine (C‐C motif chemokine ligand 18), and Treg genes (Foxp3 and IL10). Conclusion γδ T cells play multiple roles in mucosal inflammation in AR including immune surveillance and adaptive and innate immune responses.