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MicroRNAs regulating mucin type O‐glycan biosynthesis and transforming growth factor β signaling pathways in nasal mucosa of patients with chronic rhinosinusitis with nasal polyps in Northern China
Author(s) -
Xuan Lijia,
Luan Ge,
Wang Yue,
Lan Feng,
Zhang Xu,
Hao Yun,
Zheng Ming,
Wang Xiangdong,
Zhang Luo
Publication year - 2019
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.22230
Subject(s) - microrna , kegg , nasal polyps , mucin , downregulation and upregulation , signal transduction , mapk/erk pathway , medicine , pathogenesis , transforming growth factor , cancer research , immunology , transforming growth factor beta , microbiology and biotechnology , biology , gene expression , transcriptome , pathology , gene , genetics
Background Chronic rhinosinusitis with nasal polyps (CRSwNP) is a persistent sinonasal mucosa inflammatory disease. MicroRNAs (miRNAs) that are involved in the pathogenesis of CRSwNP in Northern China remain unknown. Methods A miRCURY™ LNA Array was used to analyze miRNA profiles in nasal mucosa tissues of CRSwNP patients (n = 19) and healthy controls (n = 10). Subsequent pathways were predicted by DIANA‐mirPath software. Results Five upregulated miRNAs, including miR‐210‐5p, miR‐3178, miR‐585‐3p, miR‐3146, and miR‐320e, and 19 downregulated miRNAs, including miR‐32‐3p, miR‐1299, miR‐3196, miR‐3924, and miR‐548e‐3p, were differentially expressed ( p < 0.05, fold change >2) in tissues of CRSwNP vs controls. Utilizing the Kyoto Encyclopedia of Genes and Genomes database (KEGG), which is an online database for pathway mapping, mucin type O‐glycan biosynthesis pathway was significantly enriched in upregulated miRNAs. Transforming growth factor‐beta (TGF‐β), transient receptor potential (TRP) channels, and the mitogen‐activated protein kinase (MAPK) signaling pathway were significantly linked to downregulated miRNAs. Conclusion The mucin type O‐glycan biosynthesis pathway and TGF‐β signaling pathway are regulated by miRNAs, which could be our focus in the future studies.