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Pro‐inflammatory role of transient receptor potential canonical channel 6 in the pathogenesis of chronic rhinosinusitis with nasal polyps
Author(s) -
Tang Ru,
Li ZhiPeng,
Li MingXian,
Li DaWei,
Ye HaiBo,
Su KaiMing,
Lin Hai,
Zhang WeiTian
Publication year - 2018
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.22208
Subject(s) - trpc6 , medicine , nasal polyps , pathogenesis , transient receptor potential channel , immunology , calcium channel , receptor , endocrinology , calcium
Background The pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP) has not been fully elucidated. This study sought to explore the role and mechanism of transient receptor potential canonical channel 6 (TRPC6) in the pathogenesis of CRSwNP. Methods Immunohistochemistry (IHC) was employed to evaluate TRPC6 immunolabeling. Real‐time polymerase chain reaction (PCR) was conducted to assay TRPC6, stromal interaction molecule 1 (STIM1), and calcium release‐activated calcium channel protein 1 (Orai1) messenger RNA (mRNA) levels in 70 patients with CRSwNP, including eosinophilic CRSwNP (ECRSwNP) or non‐eosinophilic CRSwNP (nECRSwNP), and 28 control subjects. The concentrations of inflammatory mediators, including interleukin (IL)‐1β, IL‐5, and IL‐25, were assayed by enzyme‐linked immunosorbent assay (ELISA). In experiments on human nasal epithelial cell (HNEC) culture and stimulation, the mean fluorescence intensity (MFI) of intracellular Ca 2+ was assayed by flow cytometry. Western blotting, real‐time PCR, and ELISA were also conducted to assess the effects and mechanisms of TRPC6 activator 1‐oleoyl‐2‐acetyl‐glycerol (OAG) and TRPC6 inhibitor 1‐[2‐(4‐methoxyphenyl)‐2‐[3‐(4‐methoxyphenyl) propoxy]ethyl‐1H‐imidazole (SKF‐96365) on HNECs. Results Upregulation of TRPC6, STIM1, and Orai1 levels was found in CRSwNP patients, particularly in those with ECRSwNP. TRPC6‐positive cells correlated positively with the numbers of eosinophils and neutrophils, respectively. Moreover, TRPC6 mRNA was positively correlated with STIM1 and Orai1 mRNA levels. The concentrations of inflammatory mediators, including IL‐1β, IL‐5, and IL‐25, were elevated in CRSwNP, especially in ECRSwNP. In cultured HNECs, TRPC6, STIM1, Orai1, Ca 2+ MFI levels, and inflammatory mediators were upregulated by lipopolysaccharide (LPS) and OAG but were inhibited by SKF‐96365. Conclusion TRPC6 plays a pro‐inflammatory role in the pathogenesis of CRSwNP via regulating Ca 2+ flow.