Premium
Effects of PM2.5 on mucus secretion and tissue remodeling in a rabbit model of chronic rhinosinusitis
Author(s) -
Zhao Renwu,
Guo Zhiqiang,
Dong Weiyang,
Deng Congrui,
Han Zhijin,
Liu Jian,
Wang Hanwei,
Zhuang Guoshun,
Zhang Ruxin
Publication year - 2018
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.22182
Subject(s) - mucus , medicine , masson's trichrome stain , pathology , pathophysiology , staining , hyperplasia , mucous membrane of nose , immunohistochemistry , goblet cell , inhalation , respiratory system , epithelium , anatomy , biology , ecology
Background According to epidemiologic studies, fine particulate matter (particulate matter ≤2.5 μm, PM2.5) is closely associated with increases in the incidence and severity of chronic rhinosinusitis (CRS). However, the role of PM2.5 in the pathophysiology of CRS remains largely unknown. In this study, we investigated the effects of PM2.5 on nasal cilia, tissue remodeling, and mucus hypersecretion in a rabbit model of CRS. Methods CRS rabbits were exposed to ambient PM2.5 via a PM2.5 inhalation exposure system. The degree of ciliary damage was evaluated using scanning electron microscopy (SEM). Histopathologic changes were determined using periodic acid‐Schiff (PAS) staining of goblet cells and Masson trichrome (MT) staining for collagen in the nasal mucosa. MUC5AC expression was assessed by immunohistochemistry. Results PM2.5 exposure substantially aggravated ciliary disruption in rabbits with CRS. PM2.5 also significantly increased goblet cell hyperplasia and collagen deposition, as reflected by PAS and MT staining. The nasal mucosa of CRS rabbits displayed markedly elevated MUC5AC expression after PM2.5 exposure. Conclusion Increases in ciliary disruption, nasal remodeling, and mucus hypersecretion may be the pathophysiologic mechanisms underlying the PM2.5‐induced aggravation of CRS.