Premium
Distinct expression of NK2 homeobox 1 (NKX2‐1) and goblet cell hyperplasia in nasal polyps with different endotypes
Author(s) -
Du Jintao,
Ba Luo,
Li Bo,
Liu Feng,
Hu Xianting,
Zhang Jie,
Liu Yafeng,
Xian Junming,
Liu Sixi,
Li Huabin
Publication year - 2017
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.21932
Subject(s) - medicine , eosinophil , nasal polyps , eosinophilia , immunohistochemistry , immunology , foxa2 , reverse transcription polymerase chain reaction , microbiology and biotechnology , transcription factor , asthma , gene expression , biology , biochemistry , gene
Background Decreased expression of airway epithelial‐specific transcription factor NK2 homeobox 1 (NKX2‐1) was associated with allergic inflammation in asthma patients. However, the expression and role of NKX2‐1 in nasal polyps (NPs) with different endotypes were undefined yet. Methods We examined the expression of key cytokines (interleukin [IL]‐4 IL‐5, IL‐13, and IL‐17A, etc.) and NKX2‐1 in NPs with different endotypes and control tissues by immunohistochemistry staining, qualitative polymerase chain reaction (qPCR), enzyme‐linked immunosorbent assay (ELISA), and Western blot analysis. Results We found 23% of chronic rhinosinusitis (CRS) with NP (CRSwNP) patients had IL‐5 + eosinophilic NPs, 40.7% of NPs were key cytokines negative NPs (KCN NPs) with less eosinophil accumulation. The expression of NKX2‐1 in IL‐5 + NPs was significantly lower than KCN NPs and normal controls ( p < 0.05). The expression of mucin 5AC (MUC5AC) and MUC5B, as well as goblet cells hyperplasia, were significantly elevated in IL‐5 + NPs, which correlated with the decreased expression of NKX2‐1 ( p < 0.05). Moreover, “SAM pointed domain containing ETS transcription factor” (SPDEF) was significantly elevated, while expression of Forkhead Box A2 (FoxA2) was significantly decreased in IL‐5 + NPs ( p < 0.05). The expression of chemokine (C‐C motif) ligand 17 (CCL17) and IL‐4 was significantly increased in IL‐5 + NPs, which was associated with eosinophil accumulation( p < 0.05). Conclusion The downregulation of NKX2‐1 in IL‐5 + NPs may be associated with tissue eosinophilia and goblet cells hyperplasia.