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Discordant frequencies of tissue‐resident and circulating CD180‐negative B cells in chronic rhinosinusitis
Author(s) -
Miljkovic Dijana,
Ou Judy,
Kirana Chandra,
Hulse Kathryn E.,
Hauben Ehud,
Psaltis Alkis,
Wormald PeterJohn,
Vreugde Sarah
Publication year - 2017
Publication title -
international forum of allergy and rhinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.503
H-Index - 46
eISSN - 2042-6984
pISSN - 2042-6976
DOI - 10.1002/alr.21924
Subject(s) - medicine , nasal polyps , chronic rhinosinusitis , flow cytometry , immune system , immunology , peripheral blood mononuclear cell , antibody , biology , biochemistry , in vitro
Background The unconventional toll‐like receptor (TLR) CD180 is implicated in chronic inflammatory diseases; however, its role in chronic rhinosinusitis (CRS) has yet to be investigated. Here we study the expression of CD180, its homologue TLR4 and myeloid differentiation factor 1 (MD1) on mucosal and systemic immune cell populations in relation to serum immunoglobulin G (IgG) levels. Methods A total of 70 patients were recruited to the study. Mucosal and peripheral blood samples were prospectively collected from CRS patients and non‐CRS controls without evidence of sinus disease. The expression of TLR4, MD1, and CD180 was investigated using qualitative real‐time polymerase chain reaction (qRT‐PCR), immunohistochemistry, and flow cytometry. Serum IgG levels were determined using enzyme‐linked immunosorbent assay (ELISA). Results CRS with nasal polyps (CRSwNP) patients had significantly increased messenger RNA (mRNA) expression of CD180 and MD1 compared to controls (5.54‐fold and 2.1‐fold, respectively, p < 0.01). B cells lacking CD180 were lower in CRSwNP tissue compared to CRS without nasal polyps (CRSsNP) and controls (21.07 ± 6.41 vs 41.61 ± 7.82 vs 40.06 ± 8.06; p < 0.01) but higher in blood (39.18 ± 8.3 vs 17.95 ± 7.82 and 12.49 ± 4.92; p ≤ 0.05). Conclusion Changes in mucosal and peripheral CD180‐expressing B cells were identified in CRSwNP patients compared to CRSsNP and controls. This suggests a role for these cells in the dysregulated immune response in these patients.